ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding.

ABSTRACT: The search for prostate cancer biomarkers has received increased attention and several DNA repair related enzymes have been linked to this dysfunction. Here we report a targeted search for single nucleotide polymorphisms (SNPs) and functional impact characterization of human ALKBH family dioxygenases related to prostate cancer. Our results uncovered a SNP of ALKBH7, rs7540, which is associated with prostate cancer disease in a statistically significantly manner in two separate cohorts, and maintained in African American men. Comparisons of molecular dynamics (MD) simulations on the wild-type and variant protein structures indicate that the resulting alteration in the enzyme induces a significant structural change that reduces ALKBH7's ability to bind its cosubstrate. Experimental spectrosc

SUBMITTER: Walker AR 

PROVIDER: S-EPMC5322872 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature