Characterization and expression analysis of genes encoding ubiquitin conjugating domain-containing enzymes in Carica papaya.

ABSTRACT:

Background

Ripening affects the quality and nutritional contents of fleshy fruits and is a crucial process of fruit development. Although several studies have suggested that ubiquitin-conjugating enzyme (E2s or UBC enzymes) are involved in the regulation of fruit ripening, little is known about the function of E2s in papaya (Carica papaya).

Methodology/principal findings

In the present study, we searched the papaya genome and identified 34 putative UBC genes, which were clustered into 17 phylogenetic subgroups. We also analyzed the nucleotide sequences of the papaya UBC (CpUBC) genes and found that both exon-intron junctions and sequence motifs were highly conserved among the phylogenetic subgroups. Using real-time PCR analysis, we also found that all the CpUBC genes were ex

SUBMITTER: Jue D 

PROVIDER: S-EPMC5322903 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature