Concordance between genomic alterations assessed by next-generation sequencing in tumor tissue or circulating cell-free DNA.

ABSTRACT: Genomic analysis of tumor tissue is the standard technique for identifying DNA alterations in malignancies. Genomic analysis of circulating tumor cell-free DNA (cfDNA) represents a relatively non-invasive method of assessing genomic alterations using peripheral blood. We compared the concordance of genomic alterations between cfDNA and tissue biopsies in this retrospective study. Twenty-eight patients with advanced solid tumors with paired next-generation sequencing tissue and cfDNA biopsies were identified. Sixty-five genes were common to both assays. Concordance was defined as the presence or absence of the identical genomic alteration(s) in a single gene on both molecular platforms. Including all aberrations, the average number of alterations per patient for tissue and cfDNA analysis wa

SUBMITTER: Chae YK 

PROVIDER: S-EPMC5323161 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature