Neuropeptide Y-mediated sex- and afferent-specific neurotransmissions contribute to sexual dimorphism of baroreflex afferent function.

ABSTRACT:

Background

Molecular and cellular mechanisms of neuropeptide-Y (NPY)-mediated gender-difference in blood pressure (BP) regulation are largely unknown.

Methods

Baroreceptor sensitivity (BRS) was evaluated by measuring the response of BP to phenylephrine/nitroprusside. Serum NPY concentration was determined using ELISA. The mRNA and protein expression of NPY receptors were assessed in tissue and single-cell by RT-PCR, immunoblot, and immunohistochemistry. NPY was injected into the nodose while arterial pressure was monitored. Electrophysiological recordings were performed on nodose neurons from rats by patch-clamp technique.

Results

The BRS was higher in female than male and ovariectomized rats, while serum NPY concentration was similar among groups. The sex-difference

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5323221 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature