Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9.

ABSTRACT: Microraft arrays have been used to screen and then isolate adherent and non-adherent cells with very high efficiency and excellent viability; however, manual screening and isolation limits the throughput and utility of the technology. In this work, novel hardware and software were developed to automate the microraft array platform. The developed analysis software identified microrafts on the array with greater than 99% sensitivity and cells on the microrafts with 100% sensitivity. The software enabled time-lapse imaging and the use of temporally varying characteristics as sort criteria. The automated hardware released microrafts with 98% efficiency and collected released microrafts with 100% efficiency. The automated system was used to examine the temporal variation in EGFP expression in c

SUBMITTER: Attayek PJ 

PROVIDER: S-EPMC5323363 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature