Functional and cardioprotective effects of simultaneous and individual activation of protein kinase A and Epac.

ABSTRACT:

Background and purpose

Myocardial cAMP elevation confers cardioprotection against ischaemia/reperfusion (I/R) injury. cAMP activates two independent signalling pathways, PKA and Epac. This study investigated the cardiac effects of activating PKA and/or Epac and their involvement in cardioprotection against I/R.

Experimental approach

Hearts from male rats were used either for determination of PKA and PKC activation or perfused in the Langendorff mode for either cardiomyocyte isolation or used to monitor functional activity at basal levels and after 30 min global ischaemia and 2 h reperfusion. Functional recovery and myocardial injury during reperfusion (LDH release and infarct size) were evaluated. Activation of PKA and/or Epac in perfused hearts was induced using cell permea

SUBMITTER: Khaliulin I 

PROVIDER: S-EPMC5323515 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature