High-fidelity Glucagon-CreER mouse line generated by CRISPR-Cas9 assisted gene targeting.

ABSTRACT:

Objective

α-cells are the second most prominent cell type in pancreatic islets and are responsible for producing glucagon to increase plasma glucose levels in times of fasting. α-cell dysfunction and inappropriate glucagon secretion occur in both type 1 and type 2 diabetes. Thus, there is growing interest in studying both normal function and pathophysiology of α-cells. However, tools to target gene ablation or activation specifically of α-cells have been limited, compared to those available for β-cells. Previous Glucagon-Cre and Glucagon-CreER transgenic mouse lines have suffered from transgene silencing, and the only available Glucagon-CreER "knock-in" mouse line results in glucagon haploinsufficiency, which can confound the interpretation of gene deletion ana

SUBMITTER: Ackermann AM 

PROVIDER: S-EPMC5323890 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature