Enteroendocrine-derived glucagon-like peptide-2 controls intestinal amino acid transport.

ABSTRACT:

Objective

Glucagon-like peptide-2 (GLP-2) is co-secreted with GLP-1 from gut endocrine cells, and both peptides act as growth factors to expand the surface area of the mucosal epithelium. Notably, GLP-2 also enhances glucose and lipid transport in enterocytes; however, its actions on control of amino acid (AA) transport remain unclear. Here we examined the mechanisms linking gain and loss of GLP-2 receptor (GLP-2R) signaling to control of intestinal amino acid absorption in mice.

Methods

Absorption, transport, and clearance of essential AAs, specifically lysine, were measured in vivo by Liquid Chromatography triple quadrupole Mass Spectrometry (LC-MS/MS) and ex vivo with Ussing chambers using intestinal preparations from Glp2r^+/+ and Glp2r<

SUBMITTER: Lee J 

PROVIDER: S-EPMC5324020 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature