Compact, cost-effective and field-portable microscope prototype based on MISHELF microscopy.

ABSTRACT: We report on a reduced cost, portable and compact prototype design of lensless holographic microscope with an illumination/detection scheme based on wavelength multiplexing, working with single hologram acquisition and using a fast convergence algorithm for image processing. All together, MISHELF (initials coming from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel) microscopy allows the recording of three Fresnel domain diffraction patterns in a single camera snap-shot incoming from illuminating the sample with three coherent lights at once. Previous implementations have proposed an illumination/detection procedure based on a tuned (illumination wavelengths centered at the maximum sensitivity of the camera detection channels) configuration but here we report on a detuned (

SUBMITTER: Sanz M 

PROVIDER: S-EPMC5324169 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature