Cinchonine induces apoptosis of HeLa and A549 cells through targeting TRAF6.

ABSTRACT:

Background

Cancer cells are known to over-express TRAF6 that is critical for both AKT and TAK1 activations. The Really Interesting New Gene (RING) domain of TRAF6 is believed to be responsible for the E3 ligase activity, ZINC fingers of TRAF6 provide critical support for the activity of the RING domain which is critical for both AKT and TAK1 activations.

Methods

We employed computational docking program to identify small molecules that could effectively and competitively bind with the RING domain of TRAF6, which is believed to be responsible for its E3 ligase activity. MTT assay and flow cytometry were employed to analyze apoptosis of cancer cells. Signaling pathways were detected using immunoprecipitation and western blotting, and immunofluorescence was pursued to assess th

SUBMITTER: Qi Y 

PROVIDER: S-EPMC5324264 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature