Segmented linear modeling of CHO fed-batch culture and its application to large scale production.

ABSTRACT: We describe a systematic approach to model CHO metabolism during biopharmaceutical production across a wide range of cell culture conditions. To this end, we applied the metabolic steady state concept. We analyzed and modeled the production rates of metabolites as a function of the specific growth rate. First, the total number of metabolic steady state phases and the location of the breakpoints were determined by recursive partitioning. For this, the smoothed derivative of the metabolic rates with respect to the growth rate were used followed by hierarchical clustering of the obtained partition. We then applied a piecewise regression to the metabolic rates with the previously determined number of phases. This allowed identifying the growth rates at which the cells underwent a metabolic shi

SUBMITTER: Ben Yahia B 

PROVIDER: S-EPMC5324675 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature