Engineered Autonomous Human Variable Domains.

ABSTRACT:

Background

The complex multi-chain architecture of antibodies has spurred interest in smaller derivatives that retain specificity but can be more easily produced in bacteria. Domain antibodies consisting of single variable domains are the smallest antibody fragments and have been shown to possess enhanced ability to target epitopes that are difficult to access using multidomain antibodies. However, in contrast to natural camelid antibody domains, human variable domains typically suffer from low stability and high propensity to aggregate.

Methods

This review summarizes strategies to improve the biophysical properties of heavy chain variable domains from human antibodies with an emphasis on aggregation resistance. Several protein engineering approaches have targeted antibody f

SUBMITTER: Nilvebrant J 

PROVIDER: S-EPMC5326600 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature