Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae.

ABSTRACT: Here we present the most comprehensive analysis of the yeast Mediator complex interactome to date. Particularly gentle cell lysis and co-immunopurification conditions allowed us to preserve even transient protein-protein interactions and to comprehensively probe the molecular environment of the Mediator complex in the cell. Metabolic ¹⁵N-labeling thereby enabled stringent discrimination between bona fide interaction partners and nonspecifically captured proteins. Our data indicates a functional role for Mediator beyond transcription initiation. We identified a large number of Mediator-interacting proteins and protein complexes, such as RNA polymerase II, general transcription factors, a large number of transcriptional activators, the SAGA complex, chromatin remodeling complexes,

SUBMITTER: Uthe H 

PROVIDER: S-EPMC5327418 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature