Femtosecond to Millisecond Dynamics of Light Induced Allostery in the Avena sativa LOV Domain.

ABSTRACT: The rational engineering of photosensor proteins underpins the field of optogenetics, in which light is used for spatiotemporal control of cell signaling. Optogenetic elements function by converting electronic excitation of an embedded chromophore into structural changes on the microseconds to seconds time scale, which then modulate the activity of output domains responsible for biological signaling. Using time-resolved vibrational spectroscopy coupled with isotope labeling, we have mapped the structural evolution of the LOV2 domain of the flavin binding phototropin Avena sativa (AsLOV2) over 10 decades of time, reporting structural dynamics between 100 fs and 1 ms after optical excitation. The transient vibrational spectra contain contributions from both the flavin chromophore and the sur

SUBMITTER: Gil AA 

PROVIDER: S-EPMC5327423 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature