Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse.

ABSTRACT: Chronic electrophysiological recordings of neuronal activity combined with two-photon Ca²⁺ imaging give access to high resolution and cellular specificity. In addition, awake drug-free experimentation is required for investigating the physiological mechanisms that operate in the brain. Here, we developed a simple head fixation platform, which allows simultaneous chronic imaging and electrophysiological recordings to be obtained from the hippocampus of awake mice. We performed quantitative analyses of spontaneous animal behaviour, the associated network states and the cellular activities in the dorsal hippocampus as well as estimated the brain stability limits to image dendritic processes and individual axonal boutons. Ca²⁺ imaging recordings revealed a relatively ster

SUBMITTER: Villette V 

PROVIDER: S-EPMC5327464 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature