Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.

ABSTRACT: For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by rea

SUBMITTER: Crescenzo-Chaigne B 

PROVIDER: S-EPMC5327478 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature