Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2.

ABSTRACT: The intestinal porcine epithelial cell line IPEC-J2, cultured under the air-liquid interface (ALI) conditions, develops remarkable morphological characteristics close to intestinal epithelial cells in vivo. Improved oxygen availability has been hypothesised to be the leading cause of this morphological differentiation. We assessed oxygen availability in ALI cultures and examined the influence of this cell culture method on glycolysis and oxidative phosphorylation in IPEC-J2 using the submerged membrane culture (SMC) and ALI cultures. Furthermore, the role of HIF-1 as mediator of oxygen availability was analysed. Measurements of oxygen tension confirmed increased oxygen availability at the medium-cell interface and demonstrated reduced oxygen extraction at the basal compartment in AL

SUBMITTER: Klasvogt S 

PROVIDER: S-EPMC5327501 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature