Repression of Toll-like receptor-4 by microRNA-149-3p is associated with smoking-related COPD.

ABSTRACT:

Background

Smoking is the leading cause of COPD. Exploring molecular markers and understanding the pathogenic mechanisms of smoking-related COPD are helpful for early clinical diagnosis and treatment of the disease. This study aims to identify specific circulating microRNAs (miRNAs) from the blood of COPD patients with a long history of smoking.

Methods

Blood samples from four different groups were collected, and miRNA microarray was performed. Differential expression of miRNAs was verified by quantitative polymerase chain reaction. In vitro, THP-1 cells were cultured and stimulated with cigarette smoke extract (CSE) or transfected with miR-149-3p inhibitor/mimics. Protein levels of Toll-like receptor 4 (TLR-4) and nuclear factor κB (NF-κB) were detected using Western blot a

SUBMITTER: Shen W 

PROVIDER: S-EPMC5327902 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature