Investigation of the fungal community structures of imported wheat using high-throughput sequencing technology.

ABSTRACT: This study introduced the application of high-throughput sequencing techniques to the investigation of microbial diversity in the field of plant quarantine. It examined the microbial diversity of wheat imported into China, and established a bioinformatics database of wheat pathogens based on high-throughput sequencing results. This study analyzed the nuclear ribosomal internal transcribed spacer (ITS) region of fungi through Illumina Miseq sequencing to investigate the fungal communities of both seeds and sieve-through. A total of 758,129 fungal ITS sequences were obtained from ten samples collected from five batches of wheat imported from the USA. These sequences were classified into 2 different phyla, 15 classes, 33 orders, 41 families, or 78 genera, suggesting a high fungal diversity ac

SUBMITTER: Shi Y 

PROVIDER: S-EPMC5328263 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature