Calcium inhibition as an intracellular signal for actin-myosin interaction.

ABSTRACT: Intracellular signaling pathways include both the activation and the inhibition of biological processes. The activation of Ca²⁺ regulation of actin-myosin interactions was examined first, whereas it took 20 years for the author to clarify the inhibitory mode by using Physarum polycephalum, a lower eukaryote. This review describes the investigation of the inhibitory mode since 1980. The inhibitory effect of Ca²⁺ on myosin was detected chemically by ATPase assays and mechanically by in vitro motility assays. The Ca²⁺-binding ability of Physarum myosin is as high as that of scallop myosin. Ca²⁺ inhibits Physarum myosin, whereas it activates scallop myosin. We cloned cDNA of the myosin heavy chain and light chains to express a hybrid of Physarum and

SUBMITTER: Kohama K 

PROVIDER: S-EPMC5328785 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature