Cronobacter sakazakii ATCC 29544 Autoaggregation Requires FliC Flagellation, Not Motility.

ABSTRACT: Cronobacter sakazakii is an opportunistic nosocomial and foodborne pathogen that causes severe infections with high morbidity and mortality rates in neonates, the elderly, and immunocompromised individuals. Little is known about the pathogenesis mechanism of this pathogen and if there are any consequences of C. sakazakii colonization in healthy individuals. In this study, we characterized the mechanisms of autoaggregation in C. sakazakii ATCC 29544 (CS29544). Autoaggregation in CS29544 occurred rapidly, within 30 min, and proceeded to a maximum of 70%. Frameshift mutations in two flagellum proteins (FlhA and FliG) were identified in two nonautoaggregating CS29544 clonal variant isolates. Strategic gene knockouts were generated to determine if structurally intact and fu

SUBMITTER: Hoeflinger JL 

PROVIDER: S-EPMC5328975 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature