Diversity and Activity of Alternative Nitrogenases in Sequenced Genomes and Coastal Environments.

ABSTRACT: The nitrogenase enzyme, which catalyzes the reduction of N₂ gas to NH₄⁺, occurs as three separate isozyme that use Mo, Fe-only, or V. The majority of global nitrogen fixation is attributed to the more efficient 'canonical' Mo-nitrogenase, whereas Fe-only and V-('alternative') nitrogenases are often considered 'backup' enzymes, used when Mo is limiting. Yet, the environmental distribution and diversity of alternative nitrogenases remains largely unknown. We searched for alternative nitrogenase genes in sequenced genomes and used PacBio sequencing to explore the diversity of canonical (nifD) and alternative (anfD and vnfD) nitrogenase amplicons in two coastal environments: the Florida Everglades and Sippewissett Marsh (MA). Genome-based s

SUBMITTER: McRose DL 

PROVIDER: S-EPMC5328986 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature