Expression of a Human Prostatic Acid Phosphatase (PAP)-IgM Fc Fusion Protein in Plants Using In vitro Tissue Subculture.

ABSTRACT: In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue subculture. Gene insertion and expression of the recombinant PAP-IgM Fc fusion protein were confirmed in each tested the first, second, and third subculture generations (SG₁, SG₂, and SG₃, respectively). Transcription levels were constantly maintained in the SG_1, SG₂, and SG₃ leaf section (top, middle, and base). The presence of the PAP-IgM Fc gene was also confirmed in each leaf section in all tested subculture generations. R

SUBMITTER: Kang YJ 

PROVIDER: S-EPMC5329016 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature