Development of a bimolecular luminescence complementation assay for RGS: G protein interactions in cells.

ABSTRACT: Cell based assessment tools and screening platforms are the preferred paradigm for small molecule identification and validation due to selectively identifying molecules with cellular activity and validation of compound activity against target proteins in their native environment. With respect to Regulator of G Protein Signaling (RGS) proteins, current cell based methodologies are either low throughput or monitor downstream signaling consequences. The increasing number of reports indicating RGS function in various disease pathogeneses highlights the need for a robust RGS inhibitor discovery and characterization paradigm. Promega's NanoBit Protein Complementation Assay utilizes NanoLuc, an engineered luciferase with enhanced luminescence characteristics which allow for both robust and kineti

SUBMITTER: Bodle CR 

PROVIDER: S-EPMC5330260 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature