Resealable, optically accessible, PDMS-free fluidic platform for ex vivo interrogation of pancreatic islets.

ABSTRACT: We report the design and fabrication of a robust fluidic platform built out of inert plastic materials and micromachined features that promote optimized convective fluid transport. The platform is tested for perfusion interrogation of rodent and human pancreatic islets, dynamic secretion of hormones, concomitant live-cell imaging, and optogenetic stimulation of genetically engineered islets. A coupled quantitative fluid dynamics computational model of glucose stimulated insulin secretion and fluid dynamics was first utilized to design device geometries that are optimal for complete perfusion of three-dimensional islets, effective collection of secreted insulin, and minimization of system volumes and associated delays. Fluidic devices were then fabricated through rapid prototyping technique

SUBMITTER: Lenguito G 

PROVIDER: S-EPMC5330806 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature