Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos.

ABSTRACT:

Purpose

Gametocyte-specific factor 1 has been shown in other species to be required for the silencing of retrotransposons via the Piwi-interacting RNA (piRNA) pathway. In this study, we aimed to isolate and assess expression of transcripts of the gametocyte-specific factor 1 (GTSF1) gene in the human female germline and in preimplantation embryos.

Methods

Complementary DNA (cDNA) libraries from human fetal ovaries and testes, human oocytes and preimplantation embryos and ovarian follicles isolated from an adult ovarian cortex biopsy were used to as templates for PCR, cloning and sequencing, and real time PCR experiments of GTSF1 expression.

Results

GTSF1 cDNA clones that covered the entire coding region were isolated from human oocytes and preimplantation embryos. GTS

SUBMITTER: Huntriss J 

PROVIDER: S-EPMC5330970 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature