Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies.

ABSTRACT: Introducing sulfhydryl groups to biomolecules to functionalize gold nanorods (GNRs) is an attractive method that involves the creation of a strong Au-S bond. Previously, we developed a facile method to functionalize GNR surfaces by thiolating antibodies using Traut's reagent. In the current study, we evaluated several methods for the introduction of thiol groups onto the surface of GNRs by using Traut's reagent, dithiotreitol (DTT), dithiolaromatic PEG6-CONHNH₂, and thiol-polyethylene glycolamine (SH-PEG-NH₂) combined with EDC reaction. We showed that the four above-mentioned thiolation methods can efficiently functionalize GNRs and simplify the functionalization procedures. The formed GNR-bioconjugates showed superior stability without compromising the biological act

SUBMITTER: Wang X 

PROVIDER: S-EPMC5331181 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature