Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia.

ABSTRACT: Cytoplasmic dyneins drive microtubule-based, minus-end directed transport in eukaryotic cells. Whereas cytoplasmic dynein 1 has been widely studied, IFT dynein has received far less attention. Here, we use fluorescence microscopy of labelled motors in living Caenorhabditis elegans to investigate IFT-dynein motility at the ensemble and single-molecule level. We find that while the kinesin composition of motor ensembles varies along the track, the amount of dynein remains relatively constant. Remarkably, this does not result in directionality changes of cargo along the track, as has been reported for other opposite-polarity, tug-of-war motility systems. At the single-molecule level, IFT-dynein trajectories reveal unexpected dynamics, including diffusion at the base, and pausing and direction

SUBMITTER: Mijalkovic J 

PROVIDER: S-EPMC5331336 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature