Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria.

ABSTRACT: We report here the behavior of naturally occurring and rationally engineered preQ1 riboswitches and their application to inducible gene regulation in mycobacteria. Because mycobacteria lack preQ1 biosynthetic genes, we hypothesized that preQ1 could be used as an exogenous nonmetabolite ligand to control riboswitches in mycobacteria. Selected naturally occurring preQ1 riboswitches were assayed and successfully drove preQ1-dependent repression of a green fluorescent protein reporter in Mycobacterium smegmatis Using structure-based design, we engineered three preQ1 riboswitches from Thermoanaerobacter tencongensis, Bacillus subtilis, and Lactobacillus rhamnosus toward achieving higher response ratios and increased repression. Assuming a steady-state model, variants

SUBMITTER: Van Vlack ER 

PROVIDER: S-EPMC5331669 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature