Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942.

ABSTRACT: Cyanobacteria are oxygenic photosynthetic prokaryotes that are able to assimilate CO₂ using solar energy and water. Metabolic engineering of cyanobacteria has suggested the possibility of direct CO₂ conversion to value-added chemicals. However, engineering of cyanobacteria has been limited due to the lack of various genetic tools for expression and control of multiple genes to reconstruct metabolic pathways for biochemicals from CO₂. Thus, we developed SyneBrick vectors as a synthetic biology platform for gene expression in Synechococcus elongatus PCC 7942 as a model cyanobacterium. The SyneBrick chromosomal integration vectors provide three inducible expression systems to control gene expression and three neutral sites for chromosomal integrations.

SUBMITTER: Kim WJ 

PROVIDER: S-EPMC5332412 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature