Effect of irradiation on DNA synthesis, NBN gene expression and chromosomal stability in cells with NBN mutations.

ABSTRACT:

Introduction

The NBN gene product is part of the MRE11/RAD50/NBN complex, which plays an essential role in genomic stability. In the study we try to answer the question what is the effect of irradiation on DNA synthesis, NBN gene expression and chromosomal stability in cells with homozygous c.657-661del, and heterozygous c.657-661del, p.I171V and p.R215W NBN gene mutations.

Material and methods

Immortalized B-lymphocytes with NBN gene mutations were X-ray irradiated at doses of 1, 2, 5 and 8 Gy/min. Radioresistant DNA synthesis rate and the percentage of cells in phase S was analyzed by ³H thymidine and BrdU incorporation assays. NBN gene expression was quantified by real-time PCR with TaqMan fluorescent probe.

Results

Inc

SUBMITTER: Nowak J 

PROVIDER: S-EPMC5332466 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature