Monocyte behaviour and tissue transglutaminase expression during experimental autoimmune encephalomyelitis in transgenic CX3CR1^gfp/gfp mice.

ABSTRACT: Leukocyte infiltration into the central nervous system (CNS) is a key pathological feature in multiple sclerosis (MS) and the MS animal model experimental autoimmune encephalomyelitis (EAE). Recently, preventing leukocyte influx into the CNS of MS patients is the main target of MS therapies and insight into cell behaviour in the circulation is needed for further elucidation of such therapies. In this study, we aimed at in vivo visualization of monocytes in a time-dependent manner during EAE. Using intravital two-photon microscopy (IVM), we imaged CX3CR1^gfp/gfp mice during EAE, visualizing CX3CR1-GFP⁺ monocytes and their dynamics in the spinal cord vasculature. Our observations showed that intraluminal crawling of CX3CR1-GFP⁺ monocytes increased even before

SUBMITTER: Chrobok NL 

PROVIDER: S-EPMC5332504 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature