Long-term tracking of budding yeast cells in brightfield microscopy: CellStar and the Evaluation Platform.

ABSTRACT: With the continuous expansion of single cell biology, the observation of the behaviour of individual cells over extended durations and with high accuracy has become a problem of central importance. Surprisingly, even for yeast cells that have relatively regular shapes, no solution has been proposed that reaches the high quality required for long-term experiments for segmentation and tracking (S&T) based on brightfield images. Here, we present CellStar, a tool chain designed to achieve good performance in long-term experiments. The key features are the use of a new variant of parametrized active rays for segmentation, a neighbourhood-preserving criterion for tracking, and the use of an iterative approach that incrementally improves S&T quality. A graphical user interface enables manual corr

SUBMITTER: Versari C 

PROVIDER: S-EPMC5332563 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature