Multi-step excitation energy transfer engineered in genetic fusions of natural and synthetic light-harvesting proteins.

ABSTRACT: Synthetic proteins designed and constructed from first principles with minimal reference to the sequence of any natural protein have proven robust and extraordinarily adaptable for engineering a range of functions. Here for the first time we describe the expression and genetic fusion of a natural photosynthetic light-harvesting subunit with a synthetic protein designed for light energy capture and multi-step transfer. We demonstrate excitation energy transfer from the bilin of the CpcA subunit (phycocyanin α subunit) of the cyanobacterial photosynthetic light-harvesting phycobilisome to synthetic four-helix-bundle proteins accommodating sites that specifically bind a variety of selected photoactive tetrapyrroles positioned to enhance energy transfer by relay. The examination of combination

SUBMITTER: Mancini JA 

PROVIDER: S-EPMC5332574 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature