17beta-estradiol Attenuates TNF-α-Induced Premature Senescence of Nucleus Pulposus Cells through Regulating the ROS/NF-κB Pathway.

ABSTRACT:

Background

Accelerated cellular senescence within the nucleus pulposus (NP) region is a common feature of disc degeneration. Our previous work indicated that TNF-α promoted NP cell senescence. Although the intervertebral disc has been reported to be an estrogen-sensitive tissue, it is unclear whether estrogen can inhibit premature senescence of NP cells.

Objective

To investigate whether 17beta-estradiol (E₂) can attenuate TNF-α-induced premature senescence of NP cells and the potential mechanism behind this regulatory process.

Methods

Isolated NP cells and intact intervertebral discs from healthy rats were cultured with or without TNF-α, E₂ or their combination. The pan estrogen receptor (ER) antagonist ICI 182780 was used to investigate the role

SUBMITTER: Li P 

PROVIDER: S-EPMC5332869 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature