Daughter Cells and Erythroid Cells Budding from PGCCs and Their Clinicopathological Significances in Colorectal Cancer.

ABSTRACT: Purpose: We previously reported that polyploid giant cancer cells (PGCCs) induced by cobalt chloride (CoCl₂) exhibit cancer stem cell properties. Daughter cells generated by PGCCs possess epithelial mesenchymal transition (EMT) phenotype changes and EMT plays an important role in cancer development and progression. This study investigated the characteristics of PGCCs from LoVo and HCT116 induced by CoCl₂ and the clinicopathological significances of PGCCs in colorectal cancer (CRC). Materials and Methods: Western blotting and immunocytochemical staining were used to compare the expression levels of EMT-related proteins between PGCCs with budding daughter cells and the control cells. In addition, tissue samples were collected from 159 patients with CRC for

SUBMITTER: Zhang D 

PROVIDER: S-EPMC5332899 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature