Abiotic and biotic factors responsible for antimonite oxidation in Agrobacterium tumefaciens GW4.

ABSTRACT: Antimonite [Sb(III)]-oxidizing bacteria can transform the toxic Sb(III) into the less toxic antimonate [Sb(V)]. Recently, the cytoplasmic Sb(III)-oxidase AnoA and the periplasmic arsenite [As(III)] oxidase AioAB were shown to responsible for bacterial Sb(III) oxidation, however, disruption of each gene only partially decreased Sb(III) oxidation efficiency. This study showed that in Agrobacterium tumefaciens GW4, Sb(III) induced cellular H₂O₂ content and H₂O₂ degradation gene katA. Gene knock-out/complementation of katA, anoA, aioA and anoA/aioA and Sb(III) oxidation and growth experiments showed that katA, anoA and aioA were essential for Sb(III) oxidation and resistance and katA was also essential for H₂O₂ resistance. Furth

SUBMITTER: Li J 

PROVIDER: S-EPMC5333119 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature