Altered gene expression and repressed markers of autophagy in skeletal muscle of insulin resistant patients with type 2 diabetes.

ABSTRACT: This case-control study was designed to investigate the gene expression profile in skeletal muscle from severely insulin resistant patients with long-standing type 2 diabetes (T2D), and to determine associated signaling pathways. Gene expression profiles were examined by whole transcriptome, strand-specific RNA-sequencing and associated signaling was determined by western blot. We identified 117 differentially expressed gene transcripts. Ingenuity Pathway Analysis related these differences to abnormal muscle morphology and mitochondrial dysfunction. Despite a ~5-fold difference in plasma insulin, we did not observe any difference in phosphorylation of AKT or AS160, although other insulin-sensitive cascades, as mTOR/4EBP1, had retained their sensitivity. Autophagy-related gene (ATG14, RB1CC

SUBMITTER: Moller AB 

PROVIDER: S-EPMC5333153 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature