Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles.

ABSTRACT: Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody responses have been analyzed by determining the titer of the humoral response using measurements such as an ELISA, neutralization, or agglutination assays. In the simplest case, sufficiently high titers of antibody against vaccine antigen(s) are sufficient to predict protection. However, antibody titer provides only a partial measure of antibody function, which is dependent on both the variable region (Fv) to bind the antigen target, and the constant region (Fc) to elicit an effector response from th

SUBMITTER: Brown EP 

PROVIDER: S-EPMC5333794 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature