Transcriptomic effects of adenosine 2A receptor deletion in healthy and endotoxemic murine myocardium.

ABSTRACT: Influences of adenosine 2A receptor (A_2AR) activity on the cardiac transcriptome and genesis of endotoxemic myocarditis are unclear. We applied transcriptomic profiling (39 K Affymetrix arrays) to identify A_2AR-sensitive molecules, revealed by receptor knockout (KO), in healthy and endotoxemic hearts. Baseline cardiac function was unaltered and only 37 A_2AR-sensitive genes modified by A_2AR KO (≥1.2-fold change, <5 % FDR); the five most induced are Mtr, Ppbp, Chac1, Ctsk and Cnpy2 and the five most repressed are Hp, Yipf4, Acta1, Cidec and Map3k2. Few canonical paths were impacted, with altered Gnb1, Prkar2b, Pde3b and Map3k2 (among others) implicating modified G protein/cAMP/PKA and cGMP/NOS signalling. Lipopolysaccharide (LPS; 20 mg/kg) challeng

SUBMITTER: Ashton KJ 

PROVIDER: S-EPMC5334196 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature