Kinetics of Angiogenic Responses in Corneal Transplantation.

ABSTRACT:

Purpose

To delineate and compare the kinetics of corneal angiogenesis after high-risk (HR) versus low-risk (LR) corneal transplantation.

Methods

In mice, intrastromal sutures were placed in the recipient graft bed 2 weeks before allogeneic transplantation to induce angiogenesis and amplify the risk of graft rejection. Control (LR) graft recipients did not undergo suture placement, and thus the host bed remained avascular at the time of transplantation. Graft hemangiogenesis and opacity scores were evaluated for 8 weeks by slit-lamp biomicroscopy. Immunohistochemistry was used to measure CD31 (blood vessels) and LYVE-1 (lymphatic vessels) cells.

Results

Biphasic kinetics were observed for hemangiogenesis in both HR and LR transplant recipients using clinical and immuno

SUBMITTER: Inomata T 

PROVIDER: S-EPMC5334361 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature