Helicobacter pylori-induced cell death is counteracted by NF-κB-mediated transcription of DARPP-32.

ABSTRACT:

Objective

DARPP-32 is a frequently amplified and overexpressed gene that promotes several oncogenic functions in gastric cancer. Herein, we investigated the relationship between Helicobacter pylori infection, proinflammatory NF-κB activation and regulation of DARPP-32.

Design

The study used in vivo and in vitro experiments. Luciferase reporter, quantitative real-time PCR, immunoblot, chromatin immunoprecipitation (ChIP), cell viability, H. pylori infection, tissue microarrays and immunohistochemical assays were used.

Results

Our results indicated that H. pylori infection increased the DARPP-32 mRNA and protein levels in gastric cancer cell lines and gastric mucosa of mice. H. pylori infection increased the activity of N

SUBMITTER: Zhu S 

PROVIDER: S-EPMC5334457 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature