Blockade of RyRs in the ER Attenuates 6-OHDA-Induced Calcium Overload, Cellular Hypo-Excitability and Apoptosis in Dopaminergic Neurons.

ABSTRACT: Calcium (Ca²⁺) dyshomeostasis induced by endoplasmic reticulum (ER) stress is an important molecular mechanism of selective dopaminergic (DA) neuron loss in Parkinson's disease (PD). Inositol 1,4,5-triphosphate receptors (IP₃Rs) and ryanodine receptors (RyRs), which are located on the ER surface, are the main endogenous Ca²⁺ release channels and play crucial roles in regulating Ca²⁺ homeostasis. However, the roles of these endogenous Ca²⁺ release channels in PD and their effects on the function and survival of DA neurons remain unknown. In this study, using a 6-hydroxydopamine (6-OHDA)-induced in vitro PD model (SN4741 Cell line), we found that 6-OHDA significantly increased cytoplasmic Ca²⁺ levels ([Ca2+]_i

SUBMITTER: Huang L 

PROVIDER: S-EPMC5334509 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature