The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel.

ABSTRACT: Viroporins are small virus-encoded ion channel proteins. Most viroporins are monovalent selective cation channels, with few showing the ability to conduct divalent cations, like calcium (Ca²⁺). Nevertheless, some viroporins are known to disrupt host cell Ca²⁺ homeostasis, which is critical for virus replication and pathogenesis. Rotavirus nonstructural protein 4 (NSP4) is an endoplasmic reticulum transmembrane glycoprotein that has a viroporin domain (VPD), and NSP4 viroporin activity elevates cytosolic Ca²⁺ in mammalian cells. The goal of this study was to demonstrate that the NSP4 VPD forms an ion channel and determine whether the channel can conduct Ca²⁺. Using planar lipid bilayer and liposome patch clamp electrophysiology, we show that a syn

SUBMITTER: Pham T 

PROVIDER: S-EPMC5335360 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature