Floc Formation Reduces the pH Stress Experienced by Microorganisms Living in Alkaline Environments.

ABSTRACT: The survival of microorganisms within a cementitious geological disposal facility for radioactive wastes heavily depends on their ability to survive the calcium-dominated, hyperalkaline conditions resulting from the dissolution of the cementitious materials. The results from this study show that the formation of flocs, composed of a complex mixture of extracellular polymeric substances (EPS), provides protection against alkaline pH values up to 13.0. The flocs were dominated by Alishewanella and Dietzia spp., producing a mannose-rich carbohydrate fraction incorporating extracellular DNA, resulting in Ca²⁺ sequestration. EPS provided a ∼10-μm thick layer around the cells within the center of the flocs, which were capable of growth at pH values of 11.0 and 11.5, main

SUBMITTER: Charles CJ 

PROVIDER: S-EPMC5335526 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature