Rapid Enrichment of Dehalococcoides-Like Bacteria by Partial Hydrophobic Separation.

ABSTRACT: Organohalide-respiring bacteria can be difficult to enrich and isolate, which can limit research on these important organisms. The goal of this research was to develop a method to rapidly (minutes to days) enrich these organisms from a mixed community. The method presented is based on the hypothesis that organohalide-respiring bacteria would be more hydrophobic than other bacteria as they dehalogenate hydrophobic compounds. The method developed tests this hypothesis by separating a portion of putative organohalide-respiring bacteria, those phylogenetically related to Dehalococcoides mccartyi, at the interface between a hydrophobic organic solvent and an aqueous medium. This novel partial separation technique was tested with a polychlorinated biphenyl-enriched sediment-free culture,

SUBMITTER: Temme HR 

PROVIDER: S-EPMC5335530 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature