Transcriptional and post-transcriptional regulation of the ionizing radiation response by ATM and p53.

ABSTRACT: In response to ionizing radiation (IR), cells activate a DNA damage response (DDR) pathway to re-program gene expression. Previous studies using total cellular RNA analyses have shown that the stress kinase ATM and the transcription factor p53 are integral components required for induction of IR-induced gene expression. These studies did not distinguish between changes in RNA synthesis and RNA turnover and did not address the role of enhancer elements in DDR-mediated transcriptional regulation. To determine the contribution of synthesis and degradation of RNA and monitor the activity of enhancer elements following exposure to IR, we used the recently developed Bru-seq, BruChase-seq and BruUV-seq techniques. Our results show that ATM and p53 regulate both RNA synthesis and stability as well

SUBMITTER: Venkata Narayanan I 

PROVIDER: S-EPMC5335570 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature