The alr-groEL1 operon in Mycobacterium tuberculosis: an interplay of multiple regulatory elements.

ABSTRACT: Threonylcarbamoyladenosine is a universally conserved essential modification of tRNA that ensures translational fidelity in cellular milieu. TsaD, TsaB and TsaE are identified as tRNA-A₃₇-threonylcarbamoyl (t⁶A)-transferase enzymes that have been reconstituted in vitro, in few bacteria recently. However, transcriptional organization and regulation of these genes are not known in any of these organisms. This study describes the intricate architecture of a complex multicistronic alr-groEL1 operon, harboring essential genes, namely tsaD, tsaB, tsaE, groES, groEL1, and alr (required for cell wall synthesis), and rimI encoding an N-α- acetyltransferase in Mycobacterium tuberculosis. Using northern blotting, RT-PCR and in vivo fluorescence assays, genes alr to groEL1 were f

SUBMITTER: Bhat AH 

PROVIDER: S-EPMC5335608 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature