Dynamics of Methylated Cytosine Flipping by UHRF1.

ABSTRACT: DNA methylation patterns, which are critical for gene expression, are replicated by DNA methyltransferase 1 (DNMT1) and ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) proteins. This replication is initiated by the recognition of hemimethylated CpG sites and further flipping of methylated cytosines (mC) by the Set and Ring Associated (SRA) domain of UHRF1. Although crystallography has shed light on the mechanism of mC flipping by SRA, tools are required to monitor in real time how SRA reads DNA and flips the modified nucleobase. To accomplish this aim, we have utilized two distinct fluorescent nucleobase surrogates, 2-thienyl-3-hydroxychromone nucleoside (3HCnt) and thienoguanosine (^thG), incorporated at different positions into hemimethylated (HM) and nonmethyla

SUBMITTER: Kilin V 

PROVIDER: S-EPMC5335914 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature