Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology.

ABSTRACT: Keloid disease (KD) is a fibroproliferative cutaneous tumour characterised by heterogeneity, excess collagen deposition and aggressive local invasion. Lack of a validated animal model and resistance to a multitude of current therapies has resulted in unsatisfactory clinical outcomes of KD management. In order to address KD from a new perspective, we applied for the first time a site-specific in situ microdissection and gene expression profiling approach, through combined laser capture microdissection and transcriptomic array. The aim here was to analyse the utility of this approach compared with established methods of investigation, including whole tissue biopsy and monolayer cell culture techniques. This study was designed to approach KD from a hypothesis-free and compartment-specific ang

SUBMITTER: Jumper N 

PROVIDER: S-EPMC5336271 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature